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KMID : 1134820080370121647
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 12 p.1647 ~ p.1653
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Cytotoxic Effect of Isolated Protein-bound Polysaccharides from Hypsizigus marmoreus Extracts by Response Surface Methodology
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Jo Jin-Ho
Jung Eun-Bong
Cho Seung-Mock
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Abstract
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This study used response surface methodology (RSM) in an effort to optimize the water extraction conditions of Hypsizigus marmoreus in order to increase cytotoxicity activity of the extract. A central composite design was applied to investigate the effects of independent variables, which included the extraction temperature (X1), extraction time (X2), the ratio of solvent to sample (X3) on dependent variables of the extracts, including extraction yield (Y1) and protein content (Y2). The estimated optimal conditions were as follows: 51.3oCextraction temperature, 8.2 hrs extraction time, and 46.7 mL/g of solvent per sample. The extract (CE) was extracted at optimal condition and crude polysaccharides (CPS) were obtained from CE by ethanol precipitation, dialysis, and freeze drying. Neutral (NPS) and acidic (APS) fraction of polysaccharides were seperated from CPS by ion chromatography. The growth inhibitory effects of the APS (0.5 mg/mL) on AGS human cancer cells were 73.97%. CPS showed the highest growth inhibitory effects on HepG2 human cancer cell at 0.5 mg/mL. However all fraction polysaccharides from Hypsizigus marmoreus showed lower than 20% growth inhibition
on SW480 human cancer cell.
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KEYWORD
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Hypsizigus marmoreus, response surface methodology, anticancer, growth inhibitory, mushroom
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